Catabolite repression mutants ofSaccharomyces cerevisiae show altered fermentative metabolism as well as cell cycle behavior in glucose-limited chemostat cultures

1998 ◽  
Vol 59 (2) ◽  
pp. 203-213 ◽  
Author(s):  
Miguel Antonio Aon ◽  
Sonia Cortassa
Keyword(s):  
Cell Cycle ◽  
Catabolite Repression ◽  
Fermentative Metabolism ◽  
Chemostat Cultures

scholarly journals Onset of DNA synthesis during the cell cycle in chemostat cultures.

1967 ◽  
Vol 57 (6) ◽  
pp. 1611-1617 ◽  
Author(s):  
H. E. Kubitschek ◽  
H. E. Bendigkeit ◽  
M. R. Loken
Keyword(s):  
Cell Cycle ◽  
Dna Synthesis ◽  
Chemostat Cultures

scholarly journals Nutritional Homeostasis in Batch and Steady-State Culture of Yeast

2004 ◽  
Vol 15 (9) ◽  
pp. 4089-4104 ◽  
Author(s):  
Alok J. Saldanha ◽  
Matthew J. Brauer ◽  
David Botstein
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Steady State ◽  
Stress Response ◽  
Stress Responses ◽  
Global Pattern ◽  
Batch Cultures ◽  
Cycle Arrest ◽  
Chemostat Cultures ◽  
Limiting Nutrient

We studied the physiological response to limitation by diverse nutrients in batch and steady-state (chemostat) cultures of S. cerevisiae. We found that the global pattern of transcription in steady-state cultures in limiting phosphate or sulfate is essentially identical to that of batch cultures growing in the same medium just before the limiting nutrient is completely exhausted. The massive stress response and complete arrest of the cell cycle that occurs when nutrients are fully exhausted in batch cultures is not observed in the chemostat, indicating that the cells in the chemostat are “poor, not starving.” Similar comparisons using leucine or uracil auxotrophs limited on leucine or uracil again showed patterns of gene expression in steady-state closely resembling those of corresponding batch cultures just before they exhaust the nutrient. Although there is also a strong stress response in the auxotrophic batch cultures, cell cycle arrest, if it occurs at all, is much less uniform. Many of the differences among the patterns of gene expression between the four nutrient limitations are interpretable in light of known involvement of the genes in stress responses or in the regulation or execution of particular metabolic pathways appropriate to the limiting nutrient. We conclude that cells adjust their growth rate to nutrient availability and maintain homeostasis in the same way in batch and steady state conditions; cells in steady-state cultures are in a physiological condition normally encountered in batch cultures.

scholarly journals Control of Nitrogen Catabolite Repression Is Not Affected by the tRNAGln-CUU Mutation, Which Results in Constitutive Pseudohyphal Growth of Saccharomyces cerevisiae

1999 ◽  
Vol 181 (8) ◽  
pp. 2472-2476 ◽  
Author(s):  
Alexander E. Beeser ◽  
Terrance G. Cooper
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Cycle ◽  
Catabolite Repression ◽  
Doubling Time ◽  
Nitrogen Availability ◽  
Nitrogen Starvation ◽  
Nitrogen Sources ◽  
Nitrogen Catabolite Repression ◽  
Pseudohyphal Growth ◽  
Specific Allele

ABSTRACT Saccharomyces cerevisiae responds to nitrogen availability in several ways. (i) The cell is able to distinguish good nitrogen sources from poor ones through a process designated nitrogen catabolite repression (NCR). Good and poor nitrogen sources do not demonstrably affect the cell cycle other than to influence the cell’s doubling time. (ii) Nitrogen starvation promotes the initiation of sporulation and pseudohyphal growth. (iii) Nitrogen starvation strongly affects the cell cycle; nitrogen-starved cells arrest in G1. A specific allele of the SUP70/CDC65tRNAGln gene (sup70-65) has been reported to be defective in nitrogen signaling associated with pseudohyphal formation, sporulation, and NCR. Our data confirm that pseudohyphal growth occurs gratuitously in sup70-65 mutants cultured in nitrogen-rich medium at 30°C. However, we find neither any defect in NCR in thesup70-65 mutant nor any alteration in the control ofYVH1 expression, which has been previously shown to be specifically induced by nitrogen starvation.

scholarly journals Physiological and Transcriptional Responses of Anaerobic Chemostat Cultures of Saccharomyces cerevisiae Subjected to Diurnal Temperature Cycles

2014 ◽  
Vol 80 (14) ◽  
pp. 4433-4449 ◽  
Author(s):  
Marit Hebly ◽  
Dick de Ridder ◽  
Erik A. F. de Hulster ◽  
Pilar de la Torre Cortes ◽  
Jack T. Pronk ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Cycle ◽  
Growth Rate ◽  
Temperature Cycling ◽  
Yeast Genome ◽  
Cycle Phase ◽  
Content Type ◽  
Diurnal Temperature ◽  
Temperature Cycles ◽  
Chemostat Cultures

ABSTRACTDiurnal temperature cycling is an intrinsic characteristic of many exposed microbial ecosystems. However, its influence on yeast physiology and the yeast transcriptome has not been studied in detail. In this study, 24-h sinusoidal temperature cycles, oscillating between 12°C and 30°C, were imposed on anaerobic, glucose-limited chemostat cultures ofSaccharomyces cerevisiae. After three diurnal temperature cycles (DTC), concentrations of glucose and extracellular metabolites as well as CO2production rates showed regular, reproducible circadian rhythms. DTC also led to waves of transcriptional activation and repression, which involved one-sixth of the yeast genome. A substantial fraction of these DTC-responsive genes appeared to respond primarily to changes in the glucose concentration. Elimination of known glucose-responsive genes revealed an overrepresentation of previously identified temperature-responsive genes as well as genes involved in the cell cycle andde novopurine biosynthesis. In-depth analysis demonstrated that DTC led to a partial synchronization of the cell cycle of the yeast populations in chemostat cultures, which was lost upon release from DTC. Comparison of DTC results with data from steady-state cultures showed that the 24-h DTC was sufficiently slow to allowS. cerevisiaechemostat cultures to acclimate their transcriptome and physiology at the DTC temperature maximum and to approach acclimation at the DTC temperature minimum. Furthermore, this comparison and literature data on growth rate-dependent cell cycle phase distribution indicated that cell cycle synchronization was most likely an effect of imposed fluctuations of the relative growth rate (μ/μmax) rather than a direct effect of temperature.

Insufficient uracil supply in fully aerobic chemostat cultures of Saccharomyces cerevisiae leads to respiro-fermentative metabolism and double nutrient-limitation

2010 ◽  
Vol 32 (7) ◽  
pp. 973-977 ◽  
Author(s):  
Thiago Olitta Basso ◽  
Marcelo Goulart Dario ◽  
Aldo Tonso ◽  
Boris Ugarte Stambuk ◽  
Andreas Karoly Gombert
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nutrient Limitation ◽  
Fermentative Metabolism ◽  
Chemostat Cultures

scholarly journals Role of the ptsN Gene Product in Catabolite Repression of the Pseudomonas putida TOL Toluene Degradation Pathway in Chemostat Cultures▿

2006 ◽  
Vol 72 (11) ◽  
pp. 7418-7421 ◽  
Author(s):  
Isabel Aranda-Olmedo ◽  
Patricia Marín ◽  
Juan L. Ramos ◽  
Silvia Marqués
Keyword(s):  
Carbon Source ◽  
Pseudomonas Putida ◽  
Gene Product ◽  
Catabolite Repression ◽  
Maximum Rate ◽  
Degradation Pathway ◽  
Toluene Degradation ◽  
Batch Cultures ◽  
Chemostat Cultures

ABSTRACT The Pseudomonas putida KT2440 TOL upper pathway is repressed under nonlimiting conditions in cells growing in chemostat with succinate as a carbon source. We show that the ptsN gene product IIANtr participates in this repression. Crc, involved in yeast extract-dependent repression in batch cultures, did not influence expression when cells were growing in a chemostat with succinate at maximum rate.

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